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The antibody is from purified rabbit serum by affinity purification by way of sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of this gene has long been noticed in lots of tumors, which results in chromosome instability, and so may well lead to tumorigenesis. This protein was discovered to affiliate with, and become associated with, the phosphorylation of NPAT protein (nuclear protein mapped towards the ATM locus), which participates in mobile-cycle regulated histone gene expression and performs a significant job in advertising and marketing mobile-cycle progression in the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode distinctive isoforms, happen to be described. Two supplemental splice variants were claimed but specific nucleotide sequence facts will not be yet obtainable.
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Blocking peptides are peptides that bind exclusively into the goal antibody and block antibody binding. These peptide commonly contains the epitope acknowledged from the antibody.
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This guide outlines further more probable optimization of cellular and immunoassay parameters to make certain the best possible success are...
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This guidebook outlines more attainable optimization of mobile and immunoassay parameters to guarantee the absolute best results are...
Antibodies sure to the blocking peptide no longer bind towards the epitope within the focus on protein. By evaluating the staining through the blocked antibody compared to the antibody alone, you can see which staining is distinct.
Target Information Cyclin E belongs towards the very conserved cyclin family members, whose members are characterized by a extraordinary periodicity in protein abundance from the cell cycle. Cyclins operate as regulators of CDK kinases. Distinct cyclins exhibit unique expression and degradation styles which lead towards the temporal coordination of read more each and every mitotic function. Cyclin E kinds a complex with and functions as being a regulatory subunit of CDK2, whose exercise is needed for cell cycle G1/S transition. This protein accumulates with the G1-S stage boundary and is also degraded as cells progress via S period.
The measurement of protein phosphorylation is a useful tool for measuring the modulation of receptor activation by both antibodies...
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